阅读说明：本技术 一种石斛直播育苗方法 (Dendrobium direct seeding seedling method ) 是由 向增旭 贾悦 王若彤 张子璇 赵家靖 廖月月 万应鸿 于 2021-09-02 设计创作，主要内容包括：本发明提供了一种石斛直播育苗方法,涉及人工育苗技术领域。本发明利用石斛原球茎直播育苗,省去了人工转接和培养基配制的步骤,大大节约了育苗成本；液体萌发培养基配方简单易得,减去植物激素的使用,减少对石斛种质稳定性的影响。本发明所述石斛原球茎直播育苗改变了传统的石斛组培快繁育苗方式,拓展了石斛栽培繁殖体系。本发明所述石斛原球茎直播育苗生长发育全过程,为石斛原球茎直播育苗提供参考。(The invention provides a direct seeding and seedling raising method for dendrobium, and relates to the technical field of artificial seedling raising. According to the invention, the dendrobium protocorm is used for direct seeding and seedling raising, so that the steps of manual transfer and culture medium preparation are omitted, and the seedling raising cost is greatly saved; the formula of the liquid germination culture medium is simple and easy to obtain, and the influence on the germplasm stability of the dendrobium is reduced by subtracting the use of plant hormones. The dendrobium protocorm direct seeding seedling method changes the traditional dendrobium tissue culture rapid propagation seedling method and expands the dendrobium cultivation propagation system. The whole process of direct seeding, seedling raising and growth development of the dendrobium protocorm provides a reference for direct seeding and seedling raising of the dendrobium protocorm.)

1. A dendrobium direct seeding seedling raising method comprises the following steps: (1) sterilizing the surface of a mature dendrobium capsule, taking out seeds, and placing the seeds in an protocorm germination culture medium for germination culture to obtain protocorms; the protocorm germination culture medium takes an N6 culture medium as a basic culture medium and also comprises 28-32 g/L sucrose;

(2) taking out the protocorm, washing off a surface culture medium, and performing sowing and direct seeding seedling management on a direct seeding seedling substrate to obtain direct seeding dendrobium seedlings; the direct seeding and seedling raising substrate comprises coconut coir, pine bark and peanut shell.

2. The direct seeding and seedling raising method according to claim 1, wherein the surface disinfection of the step (1) comprises: cleaning dust on the surface of the capsule by using the laundry powder liquid, and flushing for 30-40 min under running water; and (3) sterilizing the mixture for 30-60 seconds by using a 75% ethanol solution in an aseptic environment, washing the mixture for 3-4 times by using aseptic water, sterilizing the mixture for 6-8 min by using a 0.1% mercuric chloride solution, and washing the mixture for 3-5 times by using the aseptic water.

3. The direct seeding and seedling raising method according to claim 1, wherein the preparation method of the protocorm germination culture medium in the step (1) comprises the steps of adding 4.12g of N6 culture medium and 28-32 g of cane sugar into each 600ml of deionized water, dissolving, and then fixing the volume to 1000ml, and adjusting the pH value to 5.6-5.8.

4. The direct seeding and seedling raising method according to claim 1 or 3, wherein the germination culture of the seeds is carried out on the protocorm germination culture medium, the temperature of the germination culture is 24 +/-1 ℃, the humidity is 80 +/-5%, the illumination time is 14-16 h/d, and the illumination intensity is 2000-2500 Lx.

5. The direct seeding and seedling raising method according to claim 4, wherein the germination culture time is 25-30 days.

6. The direct seeding and seedling raising method according to claim 1, wherein the step (2) of washing out the surface nutrient solution comprises the step of taking out the protocorm and washing with deionized water for 1-2 hours.

7. The direct seeding and seedling raising method according to claim 1, wherein the preparation method of the direct seeding and seedling raising substrate in the step (2) comprises the following steps: respectively soaking coconut coir, pine bark and peanut shell in 0.5 mass percent potassium permanganate aqueous solution for 20min, cleaning with tap water for 3-4 times, draining off water, sterilizing at 121 ℃ for 20min, and mixing the sterilized coconut coir, the sterilized pine bark and the sterilized peanut shell.

8. The direct seeding and seedling raising method according to claim 7, wherein the volume ratio of the sterilized coconut coir to the sterilized pine bark to the sterilized peanut shells is 1:1: 1.

9. The direct seeding and seedling raising method according to claim 1, wherein the temperature of the direct seeding and seedling raising management in the step (2) is 23-25 ℃, the humidity is 60-70%, the illumination intensity is 1500Lx, and the illumination time is 10-12 h/d.

10. The direct seeding and seedling raising method according to claim 1 or 9, wherein the direct seeding and seedling raising management in the step (2) further comprises applying 1/4 Hoagland nutrient solution every 10 days after 2 true leaves grow out from the dendrobium protocorm.

Technical Field

The invention belongs to the technical field of artificial seedling culture, and particularly relates to a direct seeding seedling culture method for dendrobium.

Background

Herba Dendrobii (Dendrobium) is a perennial epiphytic herb of Dendrobium of Orchidaceae, and has effects of nourishing yin, clearing heat, benefiting stomach and promoting fluid production by using fresh or dry stem section as medicine, and is a traditional and rare Chinese medicinal material in China, and is known as "Jiuda Mesona" of China. Modern pharmacological research shows that dendrobine, dendrobe polysaccharide, stilbenes, amino acid and the like are substance bases for exerting pharmacological activity, and have the effects of improving human immunity, resisting tumors, resisting aging, reducing blood sugar and the like.

The dendrobium can germinate only under the condition of symbiosis with fungi in the wild environment because the seeds are fine and have no endosperm. At present, the industrialized seedling culture of dendrobium mainly adopts a tissue culture and rapid propagation method, and although the dendrobium tissue culture technology is mature, the problems of dendrobium seedling variation, quality decline, complex tissue culture process, high cost and the like in the tissue culture process restrict the development of the dendrobium industry, so that the research on the dendrobium direct seeding technology is very necessary. A large number of researches show that under the natural environment condition, direct seeding and seedling raising of dendrobium seeds are successful only under the condition of fungus symbiosis, but the germination rate is not high, and the growth of fungi is limited by the environment condition, so that the popularization of direct seeding technology is not facilitated.

Disclosure of Invention

In view of the above, the invention aims to provide a dendrobium direct seeding seedling method, which changes the traditional dendrobium tissue culture and rapid propagation seedling method, expands the dendrobium cultivation and propagation system, reduces the seedling cost and improves the seedling quality.

In order to achieve the above object, the present invention provides the following technical solutions:

the invention provides a direct seeding and seedling raising method for dendrobium, which comprises the following steps: (1) sterilizing the surface of a mature dendrobium capsule, taking out seeds, and placing the seeds in an protocorm germination culture medium for germination culture to obtain protocorms; the protocorm germination culture medium takes an N6 culture medium as a basic culture medium and also comprises 28-32 g/L sucrose;

(2) taking out the protocorm, washing off surface nutrient solution, and performing sowing and direct seeding seedling management on a direct seeding seedling substrate to obtain direct seeding dendrobium seedlings; the direct seeding and seedling raising substrate comprises coconut coir, pine bark and peanut shell.

Preferably, the surface sterilization of step (1) comprises: cleaning dust on the surface of the capsule by using the laundry powder liquid, and flushing for 30-40 min under running water; and (3) sterilizing the mixture for 30-60 seconds by using a 75% ethanol solution in an aseptic environment, washing the mixture for 3-4 times by using aseptic water, sterilizing the mixture for 6-8 min by using a 0.1% mercuric chloride solution, and washing the mixture for 3-5 times by using the aseptic water.

Preferably, the preparation method of the protocorm germination culture medium in the step (1) comprises the steps of adding 4.12g of N6 culture medium and 28-32 g of cane sugar into every 600ml of deionized water, dissolving, fixing the volume to 1000ml, and adjusting the pH value to 5.6-5.8.

Preferably, germination culture of seeds is carried out on the protocorm germination culture medium, the temperature of the germination culture is 24 +/-1 ℃, the humidity is 80 +/-5%, the illumination time is 14-16 h/d, and the illumination intensity is 2000-2500 Lx.

Preferably, the germination culture time is 25-30 days.

Preferably, the step (2) of washing away the surface nutrient solution comprises the step of taking out the protocorm and washing with deionized water for 1-2 hours.

Preferably, the preparation method of the direct seeding and seedling raising substrate in the step (2) comprises the following steps: respectively soaking coconut coir, pine bark and peanut shell in 0.5 mass percent potassium permanganate aqueous solution for 20min, washing with tap water for 3-4 times, draining off water, disinfecting at 121 ℃ for 20min, and mixing the disinfected coconut coir, the disinfected pine bark and the disinfected peanut shell in equal volume.

Preferably, the temperature of the direct seeding and seedling raising management in the step (2) is 23-25 ℃, the humidity is 60-70%, the illumination intensity is 1500Lx, and the illumination time is 10-12 h/d.

Preferably, the direct seeding and seedling management in the step (2) further comprises applying 1/4 Hoagland nutrient solution every 10 days after 2 true leaves grow out from the dendrobium protocorm.

Has the advantages that: the invention provides a direct seeding and seedling raising method for dendrobium, which is characterized in that the dendrobium protocorm is subjected to direct seeding and seedling raising, so that the steps of manual transfer and culture medium preparation are omitted, and the seedling raising cost is greatly saved; the formula of the liquid germination culture medium is simple and easy to obtain, and the influence on the germplasm stability of the dendrobium is reduced by subtracting the use of plant hormones. The liquid culture medium is adopted to develop the dendrobium seeds into protocorms, so that the germination time is shortened, the problem of difficult germination caused by poor embryo development of the direct seeding of the seeds is solved, and the success rate of the direct seeding of the dendrobium is improved. The direct seeding and seedling raising of the dendrobium protocorm changes the traditional tissue culture and rapid propagation and seedling raising mode of the dendrobium and expands a dendrobium cultivation and propagation system. The whole process of direct seeding, seedling raising and growth development of the dendrobium protocorm provides a reference for direct seeding and seedling raising of the dendrobium protocorm.

Drawings

FIG. 1 shows the situation of dendrobe protocorm in example 1 when dendrobe seeds germinate and culture for 20 days;

FIG. 2 shows the protocorm of Dendrobium nobile Lindl in example 1 after germination culture for 30 days;

FIG. 3 shows the case of fishing out the protocorm of Dendrobium nobile in example 1 with 100 mesh steel wire mesh;

FIG. 4 is a seedling tray used for direct seeding and seedling of the dendrobium protocorm in example 1;

FIG. 5 shows the sprouting growth of dendrobium protocorm in example 1 after about 20 days of direct seeding;

FIG. 6 shows the growth of one true leaf from about 40 days of the dendrobium protocorm in example 1;

FIG. 7 shows the growth of two true leaves from the dendrobium protocorm directly seeded at about 60d in example 1;

FIG. 8 shows the growth of dendrobium stem protocorm of about 90 days after direct seeding in example 1, which grows to about 1 cm;

FIG. 9 shows the growth of dendrobium protocorm growing to 3-5 cm after about 120 days of direct seeding in example 1.

Detailed Description

The invention provides a direct seeding and seedling raising method for dendrobium, which comprises the following steps: (1) sterilizing the surface of a mature dendrobium capsule, taking out seeds, and placing the seeds in an protocorm germination culture medium for germination culture to obtain protocorms; the protocorm germination culture medium takes an N6 culture medium as a basic culture medium and also comprises 30g/L sucrose;

(2) taking out the protocorm, washing off surface nutrient solution, and performing sowing and direct seeding seedling management on a direct seeding seedling substrate to obtain direct seeding dendrobium seedlings; the direct seeding and seedling raising substrate comprises coconut coir, pine bark and peanut shell.

The method comprises the steps of taking out seeds after sterilizing the surface of a mature dendrobium capsule, and placing the seeds in an protocorm germination culture medium for germination culture to obtain protocorms; the protocorm germination culture medium takes an N6 culture medium as a basic culture medium, and further comprises 28-32 g/L sucrose.

According to the invention, the surface disinfection is preferably carried out on the mature dendrobium nobile capsule which is plump, free from plant diseases and insect pests and free from rupture, and the surface disinfection preferably comprises the following steps: cleaning dust on the surface of the capsule by using the laundry powder liquid, and flushing for 30-40 min under running water; and (3) sterilizing the mixture for 30-60 seconds by using a 75% ethanol solution in an aseptic environment, washing the mixture for 3-4 times by using aseptic water, sterilizing the mixture for 6-8 min by using a 0.1% mercuric chloride solution, and washing the mixture for 3-5 times by using the aseptic water. The invention preferably utilizes a clean bench to provide the sterile environment and completes the operations of ethanol disinfection, sterile water flushing, mercuric chloride solution disinfection and sterile water flushing on the clean bench. According to the invention, after the sterile water is washed for 3-5 times, the surface moisture is preferably sucked by using filter paper.

According to the preparation method of the protocorm germination culture medium, preferably, the capsule after surface sterilization is placed on a sterile disc and peeled off by a blade, seeds are scattered into the protocorm germination culture medium, and the preparation method of the protocorm germination culture medium comprises the steps of adding 4.12g of N6 culture medium and 28-32 g of cane sugar into each 600ml of deionized water, dissolving and fixing the volume to 1000ml, adjusting the pH value to 5.6-5.8, and more preferably, the using amount of the cane sugar is 30 g. The method comprises the steps of carrying out germination culture on protocorm germination culture medium, wherein the temperature of the germination culture is preferably 24 +/-1 ℃, the humidity is preferably 80 +/-5%, the illumination time is preferably 14-16 h/d, and the illumination intensity is preferably 2000-2500 Lx; and the time for germination culture is preferably 25-30 d. In the embodiment of the invention, preferably 150-200 mL of the protocorm germination culture medium is poured into each culture bottle, and seeds are scattered into the protocorm germination culture medium; the germination stage is 10-20 days after the germination culture, and the dendrobium seeds can be observed to slowly turn into green protocorms with slightly expanded volume. The formula of the liquid germination culture medium is simple and easy to obtain, and the influence on the germplasm stability of the dendrobium is reduced by subtracting the use of plant hormones. The protocorm germination culture medium is a liquid culture medium, and the dendrobium seeds are developed into protocorms by adopting the liquid culture medium, so that the germination time is shortened, the problem of difficult germination caused by poor seed embryo development in direct seeding of the seeds is solved, and the direct seeding success rate of the dendrobium is improved.

After protocorms are obtained, the protocorms are taken out, surface nutrient solution is washed away, and seeding and direct seeding seedling management are carried out on a direct seeding seedling substrate to obtain direct seeding seedlings of dendrobium; the direct seeding and seedling raising substrate comprises coconut coir, pine bark and peanut shell.

According to the invention, a 100-mesh steel wire mesh is preferably utilized to fish out protocorms in a liquid culture medium, deionized water is used for washing for 1-2 hours, a surface nutrient solution is washed away, bacterial contamination and death caused by abundant nutrition in a natural environment are avoided, then the washed protocorms are diluted into protocorm liquid by the deionized water, the concentration is preferably 25-35 particles/1000 mu L, and 35-45 ml of the protocorms are uniformly scattered into a direct seeding seedling substrate.

The preparation method of the direct seeding and seedling raising substrate preferably comprises the following steps: respectively soaking coconut chaff, pine bark and peanut shell in 0.5 percent by mass potassium permanganate aqueous solution for 20min, cleaning the coconut chaff, the pine bark and the peanut shell by tap water for 3-4 times, draining the water, then placing the coconut chaff, the pine bark and the peanut shell into a high-pressure steam boiler at 121 ℃ for disinfection for 20min to kill worm eggs and microorganisms in the matrix, ensuring the cleanness of the matrix as much as possible, mixing the disinfected coconut chaff, the disinfected pine bark and the disinfected peanut shell in equal volumes, and placing the mixture into a seedling tray with the height of about 5-6 cm.

The sowing and direct seeding and seedling raising management of the invention are preferably carried out in an artificial climate chamber, and the culture conditions of the artificial climate chamber are preferably set as follows: the temperature is 23-25 ℃, the humidity is 60-70%, the light source is an LED lamp, the illumination intensity is 1500Lx, the illumination time is 10-12 h/d, only water is supplemented in the early stage, after 2 true leaves grow out from the dendrobium protocorm, the humidity of the matrix is kept at 50-60%, 1/4 Hoagland nutrient solution 200ml is applied once every 10 days, and watering is supplemented in the middle.

The method for direct seeding and seedling raising of dendrobium provided by the invention is described in detail below with reference to the following examples, but the method should not be construed as limiting the scope of the invention.

Example 1

Preparing dendrobium protocorm: selecting mature capsule of Dendrobium nobile with plump fruit, no plant diseases and insect pests and no rupture, cleaning dust on the surface of the capsule with laundry powder liquid, washing for 30min under running water, sterilizing for 30s with 75% ethanol on a superclean workbench, washing for 3 times with sterile water, sterilizing for 7 min with 0.1% mercuric chloride, washing for 4 times with sterile water, and sucking out surface water with filter paper. Placing the disinfected dendrobe capsule on a sterile disc, peeling the dendrobium capsule by using a blade, scattering seeds into a protocorm germination culture medium, and culturing for 30 days (figure 1 and figure 2), wherein the culture conditions are as follows: the temperature is 24 +/-1 ℃, the humidity is 80 +/-5%, the illumination time is 14h/d, and the illumination intensity is 2000 Lx.

Preparing a liquid culture medium for protocorm germination: adding 4.12g of N6 minimal medium and 30g of cane sugar into 600ml of deionized water, stirring and dissolving, adding the volume of the mixture to 1000ml, and adjusting the pH value to 5.6-5.8.

TABLE 1 growth of protocorms at different cultivation times

Incubation time	Protocorm growth conditions
		10d	The dendrobium seeds have green-turning signs, and the whole color of the culture medium is light yellow
20d	Most of the dendrobium seeds turn green, and the whole color of the culture medium is yellow green
		30d	The protocorm has slightly enlarged volume, and the whole color of the culture medium is green
40d	Large protocorm volume, partial protocorm albino

Direct seeding and seedling of protocorm: fishing out the obtained dendrobium protocorm by using a 100-mesh steel wire mesh (figure 3), pouring out the nutrient solution, slowly washing for 2 hours by using deionized water, washing out surface nutrition, and then scattering the cleaned protocorm into a direct seeding seedling substrate.

Preparing a direct seeding and seedling raising matrix: soaking three substrates of coconut coir, pine bark and peanut shell in 0.5% potassium permanganate for 20min, washing with tap water for 4 times, washing away potassium permanganate, draining off water, sterilizing in a high-pressure steam kettle at 121 ℃ for 20min to kill worm eggs and microorganisms in the substrates, ensuring the cleanness of the substrates as much as possible, uniformly mixing the substrates in an equal volume ratio, and putting the substrates into a seedling tray (figure 4) with the height of about 5-6 cm.

And (3) direct seeding and seedling management: the whole seedling culturing process is carried out in an artificial climate chamber, the culture condition of the artificial climate chamber is that the temperature is 25 ℃, the humidity is 60%, a light source is an LED lamp, the illumination intensity is 1500Lx, the illumination time is 12h/d, only water is supplemented in the early stage, the substrate humidity is kept at 50% -60%, after 2 true leaves grow out from the dendrobium protocorm, 1/4 Hoagland nutrient solution 200ml is applied once every 10 days, and watering is supplemented in the middle.

The results are shown in fig. 5-9, the dendrobium protocorm is directly sown for about 20d, the emergence sign is observed, the growing point is raised, watering is controlled at the moment, and the breeding of mixed bacteria caused by over-wetting of the substrate is avoided; about 40 days, a piece of true leaves can be observed to grow on part of protocorm, and the epiphytic relation is established between the dendrobium protocorm and the matrix, at the moment, the epiphytic ability is weaker, watering is controlled, careful management is carried out, and the epiphytic relation is prevented from being damaged by external force factors such as water flow and the like; about 60 days, two true leaves grow out of the dendrobium protocorm, and the small part of the dendrobium protocorm can observe that the lower section of the dendrobium protocorm starts to bulge and has the sign of rooting; about 90 days, the dendrobium protocorm shoots grow to about 1cm of plantlets, and have 3-4 small leaves, 1-2 roots and 0.3-0.8 cm of root length; and (3) about 120 days, when the protocorm of the dendrobium grows to be 3-5 cm, the pseudobulb is thick, the leaf area is increased, 3-5 roots are formed, and the root length is 2.0-3.0 cm.

Comparative example 1: selecting mature capsules of dendrobium with plump fruits, no plant diseases and insect pests and no rupture, peeling the capsules by using a blade, and directly scattering seeds into a direct seeding seedling substrate, wherein the preparation and seedling management of the seedling substrate are the same as those in example 1. The result shows that the dendrobium seeds have no germination signs and the direct seeding fails.

Comparative example 2: preparing a direct seeding and seedling raising matrix: soaking vermiculite in 0.5% potassium permanganate for 20min, washing with tap water for 3-4 times, washing away potassium permanganate, draining water, sterilizing in a high-pressure steam cooker at 121 ℃ for 20min to kill ova and microorganisms in the matrix to ensure the cleanness of the matrix as much as possible, and then placing the vermiculite in a seedling culture tray with the height of about 5-6 cm.

Comparative example 3: preparing a direct seeding and seedling raising matrix: soaking coconut coir in 0.5% potassium permanganate for 20min, washing with tap water for 3-4 times, washing away potassium permanganate, draining, sterilizing in a high-pressure steam cooker at 121 ℃ for 20min to kill ova and microorganisms in the matrix to ensure the cleanness of the matrix as much as possible, and then putting the coconut coir into a seedling tray with the height of about 5-6 cm.

Comparative example 4: preparing a direct seeding and seedling raising matrix: soaking the pine bark in 0.5% potassium permanganate for 20min, washing the pine bark with tap water for 3-4 times, washing away the potassium permanganate, draining the water, disinfecting the pine bark in a high-pressure steam cooker at 121 ℃ for 20min to kill worm eggs and microorganisms in the matrix to ensure the cleanness of the matrix as much as possible, and then putting the pine bark in a seedling raising tray with the height of about 5-6 cm.

Comparative example 5: preparing a direct seeding and seedling raising matrix: soaking peanut shells in 0.5% potassium permanganate for 20min, washing the peanut shells with tap water for 3-4 times, washing away the potassium permanganate, draining the water, disinfecting the peanut shells in a high-pressure steam cooker at 121 ℃ for 20min to kill ova and microorganisms in the matrix and ensure the cleanness of the matrix as much as possible, and then putting the peanut shells into a seedling raising tray with the height of about 5-6 cm.

Comparative example 6: preparing a direct seeding and seedling raising matrix: soaking coconut chaff and peanut shells in 0.5% potassium permanganate for 20min, washing the coconut chaff and the peanut shells for 3-4 times by using tap water, washing away the potassium permanganate, draining off water, disinfecting the coconut chaff and peanut shells in a high-pressure steam boiler at the temperature of 121 ℃ for 20min to kill ova and microorganisms in the matrix and ensure the cleanness of the matrix as much as possible, then uniformly mixing the matrix in an equal volume ratio, and putting the matrix into a seedling raising tray with the height of about 5-6 cm.

Comparative example 7: preparing a direct seeding and seedling raising matrix: soaking coconut coir and pine bark in 0.5% potassium permanganate for 20min, washing with tap water for 3-4 times, washing away potassium permanganate, draining, sterilizing in a high-pressure steam kettle at 121 ℃ for 20min to kill ova and microorganisms in the matrix, keeping the matrix clean as much as possible, uniformly mixing the matrix in an equal volume ratio, and placing the matrix in a seedling raising tray with the height of about 5-6 cm.

Comparative example 8: preparing a direct seeding and seedling raising matrix: soaking vermiculite and pine bark in 0.5% potassium permanganate for 20min, washing with tap water for 3-4 times, washing away potassium permanganate, draining off water, sterilizing in a high-pressure steam kettle at 121 ℃ for 20min to kill ova and microorganisms in the matrix, ensuring the cleanness of the matrix as much as possible, uniformly mixing the matrix in an equal volume ratio, and putting the matrix into a seedling raising tray with the height of about 5-6 cm.

Comparative example 9: preparing a direct seeding and seedling raising matrix: soaking vermiculite, pine bark and peanut shells in 0.5% potassium permanganate for 20min, washing the vermiculite, the pine bark and the peanut shells for 3-4 times by using tap water, washing away the potassium permanganate, draining off water, disinfecting the vermiculite, the pine bark and the peanut shells in a high-pressure steam boiler at 121 ℃ for 20min to kill ova and microorganisms in a matrix, ensuring the cleanness of the matrix as much as possible, uniformly mixing the matrix in an equal volume ratio, and putting the matrix into a seedling raising tray with the height of about 5-6 cm.

TABLE 2 Germination of Dendrobium protocorm in different media

Numbering	Matrix composition	Conditions of Germination
			Example 1	Coconut husk, pine bark and peanut shell	Can sprout 2 true leaves with more quantity
Comparative example 2	Vermiculite	Can sprout to give cotyledon, and die in late stage
			Comparative example 3	Coconut husk	Can sprout to give cotyledon, and die in late stage
Comparative example 4	Pine bark	Small part of the leaf can be differentiated into 2 true leaves, and the leaf dies gradually in later stage
			Comparative example 5	Peanut shell	Small part of the leaf can be differentiated into 2 true leaves, and the leaf dies gradually in later stage
Comparative example 6	Coconut husk and peanut shell	Can sprout 2 true leaves
			Comparative example 7	Coconut husk and pine bark	Can sprout 2 true leaves
Comparative example 8	Vermiculite and pine bark	Can sprout 2 true leaves
			Comparative example 9	Vermiculite, pine bark and peanut shell	Can sprout 2 true leaves

TABLE 3 comparison of the quality of Dendrobium officinale seedlings in different media

	Height/cm of seedling	Number of pieces/strip	Average root length/cm	Survival rate/%
					Example 1	4.85±0.28a	4.33±0.58a	2.91±0.19a	41.6±1.5a
Comparative example 6	2.63±0.91b	2.33±0.58b	1.36±0.07d	20.3±0.6c
					Comparative example 7	2.64±0.80b	2.68±0.57b	1.63±0.08c	18.3±1.5c
Comparative example 8	2.40±0.12b	2.33±0.57b	1.45±0.08cd	19.0±1.0c
					Comparative example 9	4.26±0.45a	3.33±0.58ab	2.63±0.11b	37.0±1.0b

The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.