突变的羟基苯丙酮酸双加氧酶多肽、其编码基因及用途

文档序号:173956 发布日期:2021-10-29 浏览:36次 >En<

阅读说明:本技术 突变的羟基苯丙酮酸双加氧酶多肽、其编码基因及用途 (Mutant hydroxyphenylpyruvate dioxygenase polypeptide, coding gene and application thereof ) 是由 肖翔 陶青 于彩虹 宋庆芳 鲍晓明 于 2019-09-17 设计创作,主要内容包括:提供了一种突变的羟基苯丙酮酸双加氧酶多肽、其编码基因及用途,所述突变的羟基苯丙酮酸双加氧酶多肽保留催化对羟基苯丙酮酸转化为尿黑酸或尿黑酸盐的活性,且对HPPD抑制剂除草剂的敏感性低于原始的未突变HPPD,其在对应于SEQ ID NO:1所示氨基酸序列的372位点上包含如下突变:F372A、F372G、F372V、F372P、F372S、F372T、F372C、F372M、F372Q、F372D或F372缺失。所述突变体可以赋予植物对HPPD抑制剂除草剂表现出较高的耐受性,可用于培育耐受HPPD抑制剂除草剂的植物。(Provided are a mutant hydroxyphenylpyruvate dioxygenase polypeptide which retains the activity of catalyzing the conversion of hydroxyphenylpyruvate to homogentisate or homogentisate and which is less sensitive to HPPD inhibitor herbicides than the original, unmutated HPPD, comprising the following mutations at the 372 position corresponding to the amino acid sequence shown in SEQ ID NO: 1: F372A, F372G, F372V, F372P, F372S, F372T, F372C, F372M, F372Q, F372D, or F372 are absent. The mutant can endow plants with higher tolerance to HPPD inhibitor herbicide, and can be used for cultivating plants with tolerance to the HPPD inhibitor herbicide.)

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